RFLSI Ⅲ Laser Speckle Imaging System

Overview

RFLSI Ⅲ is based on the LSCI (Laser Speckle Contrast Imaging) technology. With the advantages of its non-contact, high frame-rate, high spatial resolution, it can be used to observe and record blood perfusion of any exposed tissues or organs for microcirculation study.

This system is designed for pre-clinical microcirculation researches like ischemic stroke, lower limbs, mesentery, etc. Multi-Output includes blood perfusion images and videos (4.2 million pixels), quantified data for perfusion unit and vessel diameter.

Applications:

• Cerebral blood perfusion monitoring
• MCAO model assessment
• Cortical spreading depression observation
• Hind-limb ischemia research
• Skin burn/skin flap transplantation
• Organ microcirculation observation

  Advantages

  Reliable Data Measurement

  Stable laser output for reliable and consistent measurement over minutes, hours and days, accuracy up to 0.1 PU, easy to control variables.

  Full-Frame HD Image & Video

  High-definition camera (full-frame 2048x2048 resolution) helps you see the blood vessel endings and opens the world of microcirculation imaging.

  High Frame Rate

  A high-speed camera (up to 120 FPS) allows you to record rapid changes every second and record more details of blood vessel changes after treatment.

  Quantified Visualization Data

  Full-field monitoring, quantitative analysis. Multi-output includes vascular perfusion volume, vessel diameter, vessel angle, etc., also offline state analysis available.

  Guaranteed to Run Smoothly

  USB 3.0 compatible.Data acquisition and blood perfusion image with video generated rapidly without delay.

  Easy Operation

  Unique non-contact technology, no need for a special dark environment. Autofocus function, easy to use, get perfusion data and video in seconds.

  Successful Cases

  

  Journal: Nature Communication

  Use the laser speckle to observe changes in cerebral blood flow after lymphatic ablation, and quantitative analysis.
  Explore whether meningeal lymphatics are involved in clearing extravasated erythrocytes in CSF after SAH.

  

  Journal: Diabetes

  Use LSCI to real-time detect regional velocity and distribution of blood flow and perfused blood vessel of sciatic nerve and foot pads, and also analyze image to better evaluate the therapeutic effect of small molecule substances on diabetes.

  

  Journal: Shock

  The main purpose of the UPR is to restore ER homeostasis, and promote cell survival. It has been well established that brain I/R insult caused by transient ischemic stroke or CA/resuscitation leads to ER stress and subsequent activation of the UPR. The previous study has demonstrated that the XBP1s/HBP/O-GlcNAc axis functions in the brain, and is activated after ischemic stroke or CA (6, 8). Here, the goal was to clarify the effect of this axis on functional outcome after CA in young and aged mice.